小叶黄杨叶降血脂作用研究
作者：李勇文，杨成芳，张惠勤，李丽
【摘要】 目的研究小叶黄杨叶不同提取物的降血脂作用。方法选用系统溶媒(石油醚、醋酸乙酯、氯仿、正丁醇、水)的方法对小叶黄杨叶进行提取，观察各提取物对腹腔注射75%蛋黄乳液致高血脂症模型小鼠血清总胆固醇及甘油三酯的影响。结果小叶黄杨叶醋酸乙酯提取物的高、低两个剂量组均有显著的降低血清总胆固醇和甘油三酯的作用，小叶黄杨叶的水提取物和石油醚提取物均有显著的降低血清中总胆固醇的作用，小叶黄杨叶氯仿提取物及正丁醇提取物均无降血脂的作用。结论小叶黄杨叶醋酸乙酯提取物对高血脂症小鼠有降血脂作用。
【关键词】 小叶黄杨叶 提取物 降血脂
Abstract：ObjectiveTo study the antihyperlipidemia effects of diferent Buxus microphylla leaf extracts．MethodsDifferent extracts were extracted from Buxus microphylla leaf by systematic solvents (petroleum benzin，ethyl acetate，chloroform，n-butyl alcohol and water respectively) and the antihyperlipidemia effects of different extracts were observed on serum levels of total cholesterol and triglyceride in hyperlipidemia models，which were established through celiac injection of 75% egg-yolk emulsion. ResultsThe Buxus microphylla leaf ethyl acetate extract (high dose and low dose )had obvious effect on the levels of serum total cholesterol and triglyceride. Moreover，the effect of high-dose ethyl acetate extract was better than the other extracts. In addition, the water extract and n-butyl alcohol extract could significantly reduce serum total cholesterol, and the chloroform extract and n-butyl alcohol extract had no remarkable effect on blood lipid. ConclusionBuxus microphylla leaf ethyl -acetate extract is the active part of Buxus microphylla leaf in reducing blood lipid.
Key words：Buxus microphylla leaf Extracts Antihyperlipidemia effect
Buxus Microphylla leaf is the dry leaf from Buxus microphylla. (Family Genus). According to the “Compendium of Materia Medica”, Buxus microphylla can promote flow of “qi” and blood circulation ,eliminate pathogenic wetness ,dredge and activate the meridian.Cyclovirobuxine D is one kind of alkaloid extracted from the rhizome of Buxus Microphylla and other plants belonged to the same Genus［1］. In recent years, clinically and experimentally Cyclovirobuxine D plays a good role in anti-arrythmia, anti-myocardial Ischemia and strengthening heart, and it has protective function to the acute brain ischemia because it can pass through the blood brain barrier, suppress the formation of thrombus, improve microcycle［2-3］, however, until now no pharmacological research reports on the Buxus Microphylla leaf has been seen in the literature.In this experiment we observed the effects of system solvent extract from buxus microphylla leaf on the serum total cholesterol and triglyceride in hyperlipidemia model mice.
1 Materials and Methods
1.1 Drugs, Reagents, Animals and Instruments
1.1.1 Preparation of drugsDry Buxus microphylla leaves,from Yongfu, Guangxi Guilin, were extracted with 70 percent ethanol. The process was as follows：Firstly, 500g Buxus microphylla leaves were taken into round flask,at the same time added 3 000 ml 70% ethyl alcohol, then heated backflow, totally three times, three hours each time. After extracting the filtration, merged the filtrate. Finnally the gross extracts were concentrated to non- alcohol flavor. The gross extracts were extracted with benzine, ethyl acetate, chloroform, normal butyl alcohol respectively according to resolver polarity order from small to big ,then every extracted liquid was evaporated on the water bath to get each various part of extractions (after multiple withdraws, obtains stable fingerprint atlas). When experiment being carried on, each extraction was dissolved with 2% Twain - 80 distilled water.
1.1.2 Reagents Xue Zhi Kang capsule (Beijing University, WBL Biotech Co,LTD. Batch number: 20040513);
Total cholesterol reagent kit (Zhe Jiang Dong Ou Biological Engineering Co, LTD. Batch number:2006120060);
Triglyceride determination reagent kit (Zhe Jiang Dong Ou Biological Engineering Co, LTD. Batch number:2006120173);other reagents, analytical reagent available on market.
1.1.3 AnimalsOne hundred and thirty SPF-Kunming mice, 8-10 weeks,weighing（25.0±2.0）g,male and female, supplied by experimental animal center of Guangxi medical college（Certificate No：Gui Dong acceptance proposal 2003-0003st）; All animal experiments were carried out according to the guidelines for the care and use of the laboratory animals and were approved by the Experimental Animal Ethical Committee of Guilin Medical University.All possible efforts were taken during these experiments to avoid unnecessary suffering and to minimize the number of animals used.
1.1.4 Instruments 722-model UV-visible spectrophotometer (Shanghai Precision & Scientific Instrument Co.,LTD) ; TGL-168 high-speed desktop centrifuges (Shanghai Anting Scientific Instrument Factory);Digital two heated water bath pot (Zhengzhou Greatwall Scientific Industrial & Trade Co.,Ltd).
