癃必舒胶囊对大鼠实验性前列腺增生的影响

【摘要】  目的观察癃必舒胶囊对丙酸睾丸素致大鼠实验性前列腺增生的影响。方法大鼠去睾丸7d后，皮下注射（sc）丙酸睾丸素（3 mg/kg）制备大鼠前列腺增生症病理模型。同时连续灌胃（ig）癃必舒胶囊 （0.25，0.5，1.0 g·kg-1） 21 d,末次给药后1 h 处死大鼠,取前列腺称重,前列腺系数，并进行病理检查。结果癃必舒胶囊组前列腺湿干系数明显小于丙酸睾丸素组。病检查显示癃必舒胶囊组大鼠前列腺处于增生状态的腺体数明显低于丙酸睾丸素组，非增生状态的腺体数多于丙酸睾丸素组。结论癃必舒胶囊对大鼠实验性前列腺组织增生有明显抑制作用。

【关键词】  癃必舒胶囊 丙酸睾丸素 列腺增生

　　　Abstract:ObjectiveTo observe the effect of Longbishu capsule (LBSC) on prostatic hyperplasia in rats induced by testosterone propionate. MethodsSeven days after the male rats were castrated, testosterone propionate (3 mg/kg) was sc injected in rats. Meanwhile the rats were administered LBSC (0.25, 0.5, 1.0 mg/kg) once a day, and the treatment continued for 21d. An hour after the final administration, the rats were put to death, and the weights of prostates were measured, then count the coefficients of the prostates . The construction of prostate glands was also observed under light microscope. ResultsThe dry and the wet coefficients of the prostates in the group with LBSC capsules were obviously lower than those in the model group. The gland numbers of the prostates with hyperplasia in the LBSC group were significantly lower than those in the testesterone propionate group; the gland numbers of the prostates without hyperplasia in the LBSC group were higher than those in the testesterone propionate group. ConclusionLBSC can significantly inhibit prostatic hyperplasia in rats.

　　Key words:Longbishu Capsule (LBSC);  Testesterone propionate;  Prostatic hyperplasia
     
    良性前列腺增生（benign prostatic hyperplasis,BPH）是中老年男性常见病之一，发病率占老年男性的70%～75％［1］。因此，研究开发前列腺增生药物倍受关注。癃必舒胶囊为科迪药业有限公司研制的防治前列腺增生中药制剂。由补骨脂、益母草、金钱草、海金沙、琥珀、山慈姑等中药组成，主要以温肾化气、活血化淤、利尿通淋为主。本实验拟观察癃必舒对大鼠实验性前列腺增生的防治作用。

　　1  材料

　　1.1  药物及试剂

　　癃必舒胶囊，科迪药业有限公司研制。批号971009。丙酸睾丸素，上海第九制药厂生产，批号020506，临用时用橄榄油稀释成10 mg/ml。

　　1.2  动物wistar大鼠，体重 180～220 g，雄性，河北医科大学实验动物管理中心提供，合格证号：04057。实验前于本实验室适应7 d。

　　1.3  仪器

　　JA2003型天平：上海天平仪器厂产品。光学显微镜：日本 Olympus 产品。